YM-1L Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Sodium lactate ..................................................30.0g Mycological peptone ........................................10.0g Succinic acid.....................................................10.0g NaOH..................................................................6.0g Yeast extract........................................................5.0g Adenine.............................................................0.01g Uracil ................................................................0.01g Yeast nitrogen base solution ....................... 100.0mL Yeast Nitrogen Base Solution: Composition per 100mL: (NH4)2SO4...........................................................5.0g KH2PO4...............................................................1.0g MgSO4・7H2O ......................................................0.5g NaCl....................................................................0.1g CaCl2・2H2O.........................................................0.1g DL-Methionine...................................................0.02g DL-Tryptophan...................................................0.02g L-Histidine・HCl .................................................0.01g Inositol .............................................................2.0mg KI .....................................................................0.1mg H3BO3 ..............................................................0.5mg ZnSO4・7H2O ....................................................0.4mg MnSO4・4H2O ...................................................0.4mg Thiamine・HCl ..................................................0.4mg Pyroxidine・HCl ................................................0.4mg Niacin...............................................................0.4mg Calcium pantothenate ......................................0.4mg p-Aminobenzoic acid.......................................0.2mg Riboflavin ........................................................0.2mg FeCl3 ................................................................0.2mg Na2MoO4・4H2O ...............................................0.2mg CuSO4・5H2O ..................................................0.04mg Folic acid.......................................................... 2.0μg Biotin ............................................................... 2.0μg Preparation of Yeast Nitrogen Base Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize Preparation of Medium: Add components, ex cept yeast nitrogen base solution, to distilled/deion ized water and bring volume to 900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure? 121°C. Aseptically add 1.0L of sterile yeast nitrogen base solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks. Use: For the cultivation of Saccharomyces cerevisiae.