Z-Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ........................................................... 16.0 K2HPO4...............................................................5.0g K2SO4..................................................................2.0g KH2PO4...............................................................1.0g MgSO4・7H2O ....................................................0.05g Acetamide solution ....................................... 40.0mL pH 7.2 ± 0.2 at 25°C Acetamide Solution: Composition per 100.0mL: Acetamide .........................................................10.0g Preparation of Acetamide Solution: Add aceta mide to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Preparation of Medium: Add components, ex cept acetamide solution, to distilled/deionized water and bring volume to 960.0mL. Mix thoroughly. Au toclave for 20 min at 15 psi pressure?121°C. Cool to 70°C. Aseptically add 40.0mL sterile acetamide so lution. Mix thoroughly. Pour into sterile Petri dishes. Dry plates at 37°C for 30 min. Use: For the isolation of Pseudomonas aeruginosa from milk.